Anti-CD11B/Integrin Alpha M/ITGAM Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

PB9140

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD11B/Integrin Alpha M/ITGAM using anti-CD11B/Integrin Alpha M/ITGAM antibody (PB9140).
Lane 1: recombinant Human CD11B Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM antigen affinity purified polyclonal antibody (PB9140) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11B/Integrin Alpha M/ITGAM at approximately 45 kDa.
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IHC analysis of CD11B/Integrin Alpha M/ITGAM using anti-CD11B/Integrin Alpha M/ITGAM antibody (PB9140).
CD11B/Integrin Alpha M/ITGAM was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM Antibody (PB9140) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD11B/Integrin Alpha M/ITGAM using anti-CD11B/Integrin Alpha M/ITGAM antibody (PB9140).
CD11B/Integrin Alpha M/ITGAM was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM Antibody (PB9140) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CD11B/Integrin Alpha M/ITGAM using anti-CD11B/Integrin Alpha M/ITGAM antibody (PB9140).
CD11B/Integrin Alpha M/ITGAM was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM Antibody (PB9140) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of THP-1 cells using anti-CD11B/Integrin Alpha M/ITGAM antibody (PB9140).
Overlay histogram showing THP-1 cells stained with PB9140 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM Antibody (PB9140) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

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产品名称

Anti-CD11B/Integrin Alpha M/ITGAM Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD11B; CR 3 alpha chain; CR3A; Integrin alpha M; ITGAM; MAC 1; MAC1A; MO1A; Neutrophil adherence receptor; SLEB6

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

ITGAM

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD11b recombinant protein (Position: F17-T382). Human CD11b shares 79% and 61% amino acid (aa) sequences identity with mouse and rat CD11b, respectively.

计算分子量

127 kDa

实际分子量

127,170 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Uniprot ID

P11215

RRID

AB_3081986

文献引用格式

CD11B/Integrin Alpha M/ITGAM Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9140)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

integrin, alpha M (complement component 3 receptor 3 subunit)

蛋白名全称

Integrin alpha-M

实验方案>

实验步骤

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