| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse ANA-1 whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (A00144-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.

Western blot analysis of anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse ANA-1 whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (A00144-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa.


