Anti-CD11B/Integrin Alpha M/ITGAM Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC FCM ELISA

A00144-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of CD11B/Integrin Alpha M/ITGAM using anti-CD11B/Integrin Alpha M/ITGAM antibody (A00144-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM antigA03957-Aen affinity purified polyclonal antibody (A00144-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11B/Integrin Alpha M/ITGAM at approximately 179 kDa. The expected band size for CD11B/Integrin Alpha M/ITGAM is at 127 kDa.
all(4)
IHC analysis of CD11B/Integrin Alpha M/ITGAM using anti-CD11B/Integrin Alpha M/ITGAM antibody (A00144-1).
CD11B/Integrin Alpha M/ITGAM was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM Antibody (A00144-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Flow Cytometry analysis of U937 cells using anti-CD11B/Integrin Alpha M/ITGAM antibody (A00144-1).
Overlay histogram showing U937 cells stained with A00144-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM Antibody (A00144-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(4)
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD11B/Integrin Alpha M/ITGAM Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD11B; CR 3 alpha chain; CR3A; Integrin alpha M; ITGAM; MAC 1; MAC1A; MO1A; Neutrophil adherence receptor; SLEB6

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

ITGAM

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human CD11b recombinant protein (Position: N129-E336). Human CD11b shares 77.9% and 75.6% amino acid (aa) sequence identity with mouse and rat CD11b, respectively.

计算分子量

127 kDa

实际分子量

179 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Uniprot ID

P11215

RRID

AB_3080973

文献引用格式

CD11B/Integrin Alpha M/ITGAM Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00144-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除