Anti-CD11B/Integrin Alpha M/ITGAM Antibody (Clone#GC-9)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM3925

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-CD11B/Integrin Alpha M/ITGAM antibody (BM3925). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/ITGAM antigen affinity purified monoclonal antibody (BM3925) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD11B/Integrin Alpha M/ITGAM at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/ITGAM is at 127 kDa.
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Immunohistochemical analysis of paraffin-embedded human spleen, using CD11b Antibody.
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Immunohistochemical analysis of paraffin-embedded mouse lung, using CD11b Antibody.
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产品名称

Anti-CD11B/Integrin Alpha M/ITGAM Antibody (Clone#GC-9)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD11B; CR 3 alpha chain; CR3A; Integrin alpha M; ITGAM; MAC 1; MAC1A; MO1A; Neutrophil adherence receptor; SLEB6

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

ITGAM

克隆号

GC-9

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CD11b

计算分子量

127 kDa

实际分子量

170 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Uniprot ID

P11215

文献引用格式

CD11B/Integrin Alpha M/ITGAM Antibody (Clone#GC-9) (Boster Biological Technology, Wuhan, China. Catalog#BM3925)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Integrin alpha-M

实验方案>

实验步骤

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