Anti-P-Cadherin/CDH3 Antibody (Clone#3C9)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF

M03353

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (M03353). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-P-Cadherin/CDH3 antigen affinity purified monoclonal antibody (M03353) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-Cadherin/CDH3 at approximately 120 kDa. The expected band size for P-Cadherin/CDH3 is at 91 kDa.
all(2)
ICC/IF analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (M03353).
P-Cadherin/CDH3 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-P-Cadherin/CDH3 Antibody (M03353) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(2)

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产品名称

Anti-P-Cadherin/CDH3 Antibody (Clone#3C9)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Cadherin 3; CDH3; CDHP; HJMD; PCAD; P-cadherin

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF

基因名称

CDH3

克隆号

3C9

宿主

Mouse

抗体亚型

IgG2b

免疫原

E. coli-derived human P cadherin recombinant protein (Position: Q126-H336).

计算分子量

91 kDa

实际分子量

120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cadherins, such as CDH3, are integral membrane glycoproteins responsible for calcium-dependent cell-cell adhesion. Cadherin-3 is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy.

Uniprot ID

P22223

文献引用格式

P-Cadherin/CDH3 Antibody (Clone#3C9) (Boster Biological Technology, Wuhan, China. Catalog#M03353)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cadherin 3, type 1, P-cadherin (placental)

蛋白名全称

Cadherin-3

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实验步骤

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