Anti-P-Cadherin/CDH3 Antibody

筛选器： All WB ICC/IF FCM ELISA

• 

  Western blot analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (A03353-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-Cadherin/CDH3 antigA03957-Aen affinity purified polyclonal antibody (A03353-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-Cadherin/CDH3 at approximately 120 kDa. The expected band size for P-Cadherin/CDH3 is at 91 kDa.

• 

  ICC/IF analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (A03353-2).
  P-Cadherin/CDH3 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of A431 cells using anti-P-Cadherin/CDH3 antibody (A03353-2).
  Overlay histogram showing A431 cells stained with A03353-2 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (A03353-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-Cadherin/CDH3 antigA03957-Aen affinity purified polyclonal antibody (A03353-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-Cadherin/CDH3 at approximately 120 kDa. The expected band size for P-Cadherin/CDH3 is at 91 kDa.

• 

  ICC/IF analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (A03353-2).
  P-Cadherin/CDH3 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of A431 cells using anti-P-Cadherin/CDH3 antibody (A03353-2).
  Overlay histogram showing A431 cells stained with A03353-2 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•