Anti-P-Cadherin/CDH3 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of anti- CDH3 antibody (A03353-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human Hacat whole cell lysates.
  Use rabbit anti- CDH3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CDH3 at approximately 120KD. The expected band size for CDH3 is at 91KD.

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  ICC/IF analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (A03353-1).
  P-Cadherin/CDH3 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of A431 cells using anti-P-Cadherin/CDH3 antibody (A03353-1).
  Overlay histogram showing A431 cells stained with A03353-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti- CDH3 antibody (A03353-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human A431 whole cell lysates,
  Lane 2: human Hacat whole cell lysates.
  Use rabbit anti- CDH3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CDH3 at approximately 120KD. The expected band size for CDH3 is at 91KD.

• 

  ICC/IF analysis of P-Cadherin/CDH3 using anti-P-Cadherin/CDH3 antibody (A03353-1).
  P-Cadherin/CDH3 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-1) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of A431 cells using anti-P-Cadherin/CDH3 antibody (A03353-1).
  Overlay histogram showing A431 cells stained with A03353-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P-Cadherin/CDH3 Antibody (A03353-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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