Anti-E-cadherin/CDH1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF ELISA

PB9561

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-E-cadherin/CDH1 antigA03957-Aen affinity purified polyclonal antibody (PB9561) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for E-cadherin/CDH1 at approximately 130 kDa. The expected band size for E-cadherin/CDH1 is at 97 kDa.
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IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
E-cadherin/CDH1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
E-cadherin/CDH1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
E-cadherin/CDH1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
E-cadherin/CDH1 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
E-cadherin/CDH1 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
E-cadherin/CDH1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-E-cadherin/CDH1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, ELISA

基因名称

CDH1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

计算分子量

97 kDa

实际分子量

130 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Uniprot ID

P12830

RRID

AB_3082161

文献引用格式

E-cadherin/CDH1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9561)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cadherin 1, type 1, E-cadherin (epithelial)

蛋白名全称

Cadherin-1

实验方案>

实验步骤

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