Anti-Caveolin-1/CAV1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM

PB9165

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Caveolin-1/CAV1 antibody (PB9165). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: rat ovary tissue lysates,
Lane 7: rat heart tissue lysates,
Lane 8: mouse ovary tissue lysates,
Lane 9: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caveolin-1/CAV1 antigen affinity purified polyclonal antibody (PB9165) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caveolin-1/CAV1 at approximately 22 kDa. The expected band size for Caveolin-1/CAV1 is at 20 kDa.
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IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human meningeoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165).
Caveolin-1/CAV1 was detected in frozen section of human placenta tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of U87 cells using anti-Caveolin-1/CAV1 antibody (PB9165).
Overlay histogram showing U87 cells stained with PB9165 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1/CAV1 Antibody (PB9165, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Caveolin-1/CAV1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BSCL3; CAV; CAV1; Caveolin 1; Caveolin-1; CGL3; MSTP085; VIP21

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM

基因名称

CAV1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively.

计算分子量

20 kDa

实际分子量

22 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CAV1( Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms(alpha and beta) are encoded by a single transcript from this gene.

Uniprot ID

Q03135

RRID

AB_3081999

文献引用格式

Caveolin-1/CAV1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9165)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

caveolin 1, caveolae protein, 22kDa

蛋白名全称

Caveolin-1

实验方案>

实验步骤

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