Anti-Caveolin-1/CAV1 Antibody (Clone#12C7)

Mouse monoclonal antibody

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筛选器： All IF WB IHC

M00179-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IF analysis using Anti-CAV1 antibody (M00179-1) detected in paraffin-embedded section of human lung cancer tissue. The tissue section were stained using the Fluoro488 Anti-Mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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IF analysis using Anti-CAV1 antibody (M00179-1) detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Fluoro488 Anti-Mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
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Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human U-87 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Caveolin-1/CAV1 antigen affinity purified monoclonal antibody (M00179-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caveolin-1/CAV1 at approximately 22 kDa. The expected band size for Caveolin-1/CAV1 is at 20 kDa.
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IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)
IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (M00179-1).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human placenta cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Caveolin-1/CAV1 Antibody (M00179-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(8)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Caveolin-1/CAV1 Antibody (Clone#12C7)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BSCL3; CAV; CAV1; Caveolin 1; Caveolin-1; CGL3; MSTP085; VIP21

产品类型

Monoclonal

检验物种

human

应用范围

IF, WB, IHC

基因名称

CAV1

克隆号

12C7

宿主

Mouse

抗体亚型

IgG2a

免疫原

E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat Caveolin-1, respectively.

计算分子量

20 kDa

实际分子量

22 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CAV1( Caveolin-1) is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms(alpha and beta) are encoded by a single transcript from this gene.

Uniprot ID

Q03135

RRID

AB_3081755

文献引用格式

Caveolin-1/CAV1 Antibody (Clone#12C7) (Boster Biological Technology, Wuhan, China. Catalog#M00179-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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