Anti-NF-L/NEFL Antibody (Clone#OTI3G2)

Mouse monoclonal antibody

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筛选器： All IHC WB

M02482-6

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-NEFL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M02482-6)
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Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M02482-6)
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Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M02482-6)
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Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NEFL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M02482-6)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFL.
all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NF-L/NEFL Antibody (Clone#OTI3G2)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

68 kDa neurofilament protein; CMT1F; CMT2E; NEFL; NF; NF L; NF68; NFL; NF-L

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

NEFL

克隆号

OTI3G2

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 1-360 of human NEFL (NP_006149) produced in E.coli.

计算分子量

62 kDa

实际分子量

61.3 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Uniprot ID

P07196

文献引用格式

NF-L/NEFL Antibody (Clone#OTI3G2) (Boster Biological Technology, Wuhan, China. Catalog#M02482-6)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

neurofilament light

蛋白名全称

Neurofilament light polypeptide

实验方案>

实验步骤

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