Anti-NF-L/NEFL Antibody (Clone#NR4)

Mouse monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

BM1295

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti- NEFL antibody (BM1295). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SH-SY5Y whole cell lysates,
Lane 2: U251 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
Use mouse anti- NEFL 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for NEFL at approximately 72KD. The expected band size for NEFL is at 62KD.
all(3)
IHC analysis using anti- NEFL antibody (BM1295). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3)
IHC analysis using anti- NEFL antibody (BM1295). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NF-L/NEFL Antibody (Clone#NR4)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

68 kDa neurofilament protein; CMT1F; CMT2E; NEFL; NF; NF L; NF68; NFL; NF-L

产品类型

Monoclonal

检验物种

human, mouse, rat, pig

应用范围

WB, IHC

基因名称

NEFL

克隆号

NR4

宿主

Mouse

抗体亚型

IgG1

免疫原

Pig spinal cord.

计算分子量

62 kDa

实际分子量

72 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

纯化方式

Ascites

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM) and 200 kD(NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68(NF68), also called Neurofilament Protein, Light Chain(NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E(CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Uniprot ID

P07196

文献引用格式

NF-L/NEFL Antibody (Clone#NR4) (Boster Biological Technology, Wuhan, China. Catalog#BM1295)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

neurofilament, light polypeptide

蛋白名全称

Neurofilament light polypeptide

实验方案>

实验步骤

引用文献(0)>

清除