| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:20 |

Western blot analysis of anti- ATG9A Antibody (BM4543). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: HELA whole cell lysates.
Use rabbit anti- ATG9A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG9A at approximately 100KD. The expected band size for ATG9A is at 94KD.

Western blot analysis of anti- ATG9A Antibody (BM4543). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: HELA whole cell lysates.
Use rabbit anti- ATG9A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG9A at approximately 100KD. The expected band size for ATG9A is at 94KD.






