Anti-ATG9A Antibody

筛选器： All WB ICC/IF FCM ELISA

• 

  Western blot analysis of ATG9A using anti-ATG9A antibody (A03757-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human HepG2 whole cell lysates,
  Lane 2: Human A549 whole cell lysates,
  Lane 3: Human A375 whole cell lysates,
  Lane 4: Human MCF-7 whole cell lysates,
  Lane 5: Human K562 whole cell lysates,
  Lane 6: Mouse testis tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG9A antigen affinity purified polyclonal antibody (A03757-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG9A at approximately 100-110 kDa. The expected band size for ATG9A is at 94 kDa.

• 

  ICC/IF analysis of ATG9A using anti-ATG9A antibody (A03757-2).
  ATG9A was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ATG9A Antibody (A03757-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of U87 cells using anti-ATG9A antibody (A03757-2).
  Overlay histogram showing U87 cells stained with A03757-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG9A Antibody (A03757-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of ATG9A using anti-ATG9A antibody (A03757-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human HepG2 whole cell lysates,
  Lane 2: Human A549 whole cell lysates,
  Lane 3: Human A375 whole cell lysates,
  Lane 4: Human MCF-7 whole cell lysates,
  Lane 5: Human K562 whole cell lysates,
  Lane 6: Mouse testis tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG9A antigen affinity purified polyclonal antibody (A03757-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG9A at approximately 100-110 kDa. The expected band size for ATG9A is at 94 kDa.

• 

  ICC/IF analysis of ATG9A using anti-ATG9A antibody (A03757-2).
  ATG9A was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ATG9A Antibody (A03757-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of U87 cells using anti-ATG9A antibody (A03757-2).
  Overlay histogram showing U87 cells stained with A03757-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG9A Antibody (A03757-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

•