| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:20 |
| Flow Cytometry (FCM): | 1:20 |

Western blot analysis of anti-LaminA/C antibody (BM4105). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SK-OV-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LaminA/C antigen affinity purified monoclonal antibody (BM4105) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LaminA/C at approximately 70-74 kDa. The expected band size for LaminA/C is at 74 kDa.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human esophageal cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human pancreas cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Immunofluorescent analysis using the Antibody.

Western blot analysis of anti-LaminA/C antibody (BM4105). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SK-OV-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LaminA/C antigen affinity purified monoclonal antibody (BM4105) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LaminA/C at approximately 70-74 kDa. The expected band size for LaminA/C is at 74 kDa.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human esophageal cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human pancreas cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lamin A/C using anti-Lamin A/C antibody (BM4105).
Lamin A/C was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-Lamin A/C Antibody (BM4105) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Immunofluorescent analysis using the Antibody.














