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Anti-CDK1 Antibody (Clone#BOH-3)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF IP

BM4062

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-CDK1 antibody (BM4062). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human A549 whole cell lysates,
    Lane 2: human 293T whole cell lysates,
    Lane 3: human Caco-2 whole cell lysates,
    Lane 4: human K562 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK1 antigen affinity purified monoclonal antibody (BM4062) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK1 at approximately 30 kDa. The expected band size for CDK1 is at 34 kDa.

    all(7)
  • IHC analysis of CDK1 using anti-CDK1 antibody (BM4062) .
    CDK1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CDK1 Antibody (BM4062) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of CDK1 using anti-CDK1 antibody (BM4062) .
    CDK1 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. The tissue section was incubated with rabbit anti-CDK1 Antibody (BM4062) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of CDK1 using anti-CDK1 antibody (BM4062) .
    CDK1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-CDK1 Antibody (BM4062) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of CDK1 using anti-CDK1 antibody (BM4062) .
    CDK1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CDK1 Antibody (BM4062) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • ICC/IF analysis of CDK1 using anti-CDK1 antibody (BM4062) and anti-Beta Tubulin antibody (M01857-3).
    CDK1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CDK1 Antibody (BM4062) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

    all(7)

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产品简介>

产品名称
Anti-CDK1 Antibody (Clone#BOH-3)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
CDC2; CDC28A; CDK1; Cell division protein kinase 1; Cyclin dependent kinase 1; p34 protein kinase
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF, IP
基因名称
CDK1
克隆号
BOH-3
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human CDK1
计算分子量
34 kDa
实际分子量
34 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
The cyclin-dependent protein kinases(CDKs) regulate major cell cycle transitions in eukaryotic cells. CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR(EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIRE motif found in prototypic CDC2 kinases. CDC2L1 is referred as PITSLRE B, based on the amino acid sequence of the region corresponding to the conserved CDC2 PSTAIRE box.
Uniprot ID
P06493  
文献引用格式
CDK1 Antibody (Clone#BOH-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4062)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Cyclin-dependent kinase 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20

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    Anti-CDK1 Antibody (Clone#BOH-3)

    筛选器: All WB IHC ICC/IF IP

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