| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:20 |

Western blot analysis of anti-YY1 antibody (BM4049). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YY1 antigen affinity purified monoclonal antibody (BM4049) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YY1 at approximately 68 kDa. The expected band size for YY1 is at 45 kDa.

Immunohistochemical analysis of paraffin-embedded human bladder using YY1 Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis of Hela cells, using YY1 Antibody.

Western blot analysis of anti-YY1 antibody (BM4049). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YY1 antigen affinity purified monoclonal antibody (BM4049) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YY1 at approximately 68 kDa. The expected band size for YY1 is at 45 kDa.

Immunohistochemical analysis of paraffin-embedded human bladder using YY1 Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis using the Antibody.

Immunofluorescent analysis of Hela cells, using YY1 Antibody.










