| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:50 |

Western blot analysis of anti-p21/CDKN1A antibody (BM3990). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human SiHa whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p21/CDKN1A antigen affinity purified polyclonal antibody (BM3990) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p21/CDKN1A at approximately 21 kDa. The expected band size for p21/CDKN1A is at 18 kDa.

Western blot analysis of anti-p21/CDKN1A antibody (BM3990). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human SiHa whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p21/CDKN1A antigen affinity purified polyclonal antibody (BM3990) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p21/CDKN1A at approximately 21 kDa. The expected band size for p21/CDKN1A is at 18 kDa.
