| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |

Western blot analysis of anti- CDK6 antibody (BM1616). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates.
Use mouse anti- CDK6 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CDK6 at approximately 37KD. The expected band size for CDK6 is at 37KD.

Western blot analysis of anti- CDK6 antibody (BM1616). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates.
Use mouse anti- CDK6 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CDK6 at approximately 37KD. The expected band size for CDK6 is at 37KD.


