Anti-CDK6 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All IHC WB

PB1047

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

IHC analysis of CDK6 using anti-CDK6 antibody (PB1047).
CDK6 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK6 Antibody (PB1047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Western blot analysis of CDK6 using anti-CDK6 antibody (PB1047). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK6 antigen affinity purified polyclonal antibody (PB1047) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK6 at approximately 37 kDa. The expected band size for CDK6 is at 37 kDa.
all(4)
IHC analysis of CDK6 using anti-CDK6 antibody (PB1047).
CDK6 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK6 Antibody (PB1047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of CDK6 using anti-CDK6 antibody (PB1047).
CDK6 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CDK6 Antibody (PB1047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CDK6 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CDK6; Cell division protein kinase 6; cyclin dependent kinase 6; PLSTIRE

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

CDK6

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Cdk6 identical to the related mouse sequence.

计算分子量

37 kDa

实际分子量

37 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cell division protein kinase 6, also called Plstire, is an enzyme that in humans is encoded by the CDK6 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. Radiation hybrid analysis and inclusion within a mapped clone place the CDK6 gene at 7q21. Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation and promotes G1/S transition. This gene also involved in initiation and maintenance of cell cycle exit during cell differentiation. It prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types. In addition, CDK6 plays a role in promoting the proliferation of beta-cells in pancreatic islets of Langerhans.

Uniprot ID

Q00534

文献引用格式

CDK6 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1047)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin-dependent kinase 6

蛋白名全称

Cyclin-dependent kinase 6

实验方案>

实验步骤

引用文献(0)>

清除