Anti-ATG3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

A01768-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ATG3 using anti-ATG3 antibody (A01768-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG3 antigen affinity purified polyclonal antibody (A01768-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG3 at approximately 36-40 kDa. The expected band size for ATG3 is at 36 kDa.
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产品简介>

产品名称

Anti-ATG3 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

APG3; APG3 LIKE; APG3L; ATG3; Autophagy related protein 3; hApg3; PC3 96; Protein PC3 96

产品类型

Polyclonal

检验物种

human, mouse, monkey

应用范围

基因名称

ATG3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Apg3 recombinant protein (Position: M1-T244). Human Apg3 shares 96.7% and 96.3% amino acid (aa) sequence identity with mouse and rat Apg3, respectively.

计算分子量

36 kDa

实际分子量

38 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

This ATG3 gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. And the protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Uniprot ID

Q9NT62

文献引用格式

ATG3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01768-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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