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Anti-ATG3 Antibody (Clone#OTI2C12)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF FCM

M01768-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

    all(15)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3(Cat# M01768-1).

    all(15)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01768-1)

    all(15)
  • Anti-ATG3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3 .

    all(15)
  • Immunofluorescent staining of HeLa cells using anti-ATG3 mouse monoclonal antibody.

    all(15)
  • HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATG3 antibody, and then analyzed by flow cytometry.

    all(15)

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产品简介>

产品名称
Anti-ATG3 Antibody (Clone#OTI2C12)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
APG3; APG3 LIKE; APG3L; ATG3; Autophagy related protein 3; hApg3; PC3 96; Protein PC3 96
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
ATG3
克隆号
OTI2C12
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human ATG3(NP_057393) produced in HEK293 cell.
计算分子量
36 kDa
实际分子量
35.7 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
0.39 mg/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM]
Uniprot ID
Q9NT62  
文献引用格式
ATG3 Antibody (Clone#OTI2C12) (Boster Biological Technology, Wuhan, China. Catalog#M01768-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Ubiquitin-like-conjugating enzyme ATG3
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:150
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50~100
Flow cytometry (FCM):1:100

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    Anti-ATG3 Antibody (Clone#OTI2C12)

    筛选器: All WB IHC ICC/IF FCM

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