Anti-p57Kip2/CDKN1C Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A01244-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: THP-1 whole cell lysates,
Lane 5: Raji whole cell lysates,
Lane 6: SW620 whole cell lysates,
Lane 7: Caco-2 whole cell lysates,
Lane 8: HEK293 whole cell lysates,
Lane 9: PC-12 whole cell lysates,
Lane 10: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p57Kip2/CDKN1C antigen affinity purified polyclonal antibody (A01244-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p57Kip2/CDKN1C at approximately 30 kDa/57 kDa. The expected band size for p57Kip2/CDKN1C is at 32 kDa.
all(6)
IHC analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-2).
p57Kip2/CDKN1C was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p57Kip2/CDKN1C Antibody (A01244-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-2).
p57Kip2/CDKN1C was detected in a paraffin-embedded section of human cervical cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p57Kip2/CDKN1C Antibody (A01244-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-2).
p57Kip2/CDKN1C was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p57Kip2/CDKN1C Antibody (A01244-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-2).
p57Kip2/CDKN1C was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p57Kip2/CDKN1C Antibody (A01244-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-2).
p57Kip2/CDKN1C was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p57Kip2/CDKN1C Antibody (A01244-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-p57Kip2/CDKN1C Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

BWCR; BWS; CDKN1C; KIP2; p57; p57Kip2; WBS

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

CDKN1C

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human p57 Kip2/CDKN1C, identical to the related mouse sequence.

计算分子量

32 kDa

实际分子量

30 kDa/57 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cyclin-dependent kinase inhibitor 1C (p57, Kip2), also known as CDKN1C, is a protein which in humans is encoded by the CDKN1C imprinted gene. It is mapped to 11p15.4. This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.

Uniprot ID

P49918

文献引用格式

p57Kip2/CDKN1C Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01244-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin dependent kinase inhibitor 1C

蛋白名全称

Cyclin-dependent kinase inhibitor 1C

实验方案>

实验步骤

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