| Western blot (WB): | 1:500-2000 |

Western blot analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p57Kip2/CDKN1C antigen affinity purified polyclonal antibody (A01244-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p57Kip2/CDKN1C at approximately 30 kDa/57 kDa. The expected band size for p57Kip2/CDKN1C is at 32 kDa.

Western blot analysis of p57Kip2/CDKN1C using anti-p57Kip2/CDKN1C antibody (A01244-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p57Kip2/CDKN1C antigen affinity purified polyclonal antibody (A01244-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p57Kip2/CDKN1C at approximately 30 kDa/57 kDa. The expected band size for p57Kip2/CDKN1C is at 32 kDa.
