| Western blot (WB): | 1:500-2000 |

Western blot analysis of ATG13 using anti-ATG13 antibody (PB0523). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HEPA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG13 antigen affinity purified polyclonal antibody (PB0523) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG13 at approximately 56 kDa. The expected band size for ATG13 is at 57 kDa.

Western blot analysis of ATG13 using anti-ATG13 antibody (PB0523). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: HEPA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG13 antigen affinity purified polyclonal antibody (PB0523) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG13 at approximately 56 kDa. The expected band size for ATG13 is at 57 kDa.
