| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:20-100 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |

Western blot analysis of GAP43 using anti-GAP43 antibody (PA1037). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U87 whole cell lysates at 40ug,
Lane 2: Rat Brain tissue lysates at 50ug,
Lane 3: Mouse Brain tissue lysates at 50ug.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (PA1037) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAP43 at approximately 43 kDa. The expected band size for GAP43 is at 25 kDa.

IHC analysis of GAP43 using anti-GAP43 antibody (PA1037).
GAP43 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GAP43 Antibody (PA1037) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of GAP43 using anti-GAP43 antibody (PA1037).
GAP43 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-GAP43 Antibody (PA1037) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of GAP43 using anti-GAP43 antibody (PA1037).
GAP43 was detected in a paraffin-embedded section of human meningeoma tissue. The tissue section was incubated with rabbit anti-GAP43 Antibody (PA1037) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of GAP43 using anti-GAP43 antibody (PA1037). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U87 whole cell lysates at 40ug,
Lane 2: Rat Brain tissue lysates at 50ug,
Lane 3: Mouse Brain tissue lysates at 50ug.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (PA1037) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAP43 at approximately 43 kDa. The expected band size for GAP43 is at 25 kDa.

IHC analysis of GAP43 using anti-GAP43 antibody (PA1037).
GAP43 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GAP43 Antibody (PA1037) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of GAP43 using anti-GAP43 antibody (PA1037).
GAP43 was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-GAP43 Antibody (PA1037) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of GAP43 using anti-GAP43 antibody (PA1037).
GAP43 was detected in a paraffin-embedded section of human meningeoma tissue. The tissue section was incubated with rabbit anti-GAP43 Antibody (PA1037) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.



