Anti-ATG4B Antibody (Clone#OTI1A3)

Mouse monoclonal antibody

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筛选器： All WB IHC

M02885-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-ATG4B monoclonal antibody (1:500).
all(3)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG4B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG4B (Cat# M02885-1)(1:2000).
all(3)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02885-1) (1:2000)
all(3)

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产品简介>

产品名称

Anti-ATG4B Antibody (Clone#OTI1A3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

APG4B; ATG4B; AUTL1; Autophagin 1; Cysteine protease ATG4B; hAPG4B; KIAA0943

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

ATG4B

克隆号

OTI1A3

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full length human recombinant protein of human ATG4B (NP_847896) produced in HEK293T Cell.

计算分子量

44 kDa

实际分子量

42.4 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot ID

Q9Y4P1

文献引用格式

ATG4B Antibody (Clone#OTI1A3) (Boster Biological Technology, Wuhan, China. Catalog#M02885-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

autophagy related 4B cysteine peptidase

蛋白名全称

Cysteine protease ATG4B

实验方案>

实验步骤

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