Anti-ATG4B Antibody

筛选器： All WB FCM

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  Western blot analysis of anti- ATG4B antibody (A02885-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human Ramos whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: rat brain tissue lysates,
  Lane 5: mouse brain tissue lysates.
  Use rabbit anti- ATG4B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ATG4B at approximately 48-50KD. The expected band size for ATG4B is at 44KD.

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  Flow Cytometry analysis of RT4 cells using anti-ATG4B antibody (A02885-2).
  Overlay histogram showing RT4 cells stained with A02885-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4B Antibody (A02885-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti- ATG4B antibody (A02885-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human Ramos whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: rat brain tissue lysates,
  Lane 5: mouse brain tissue lysates.
  Use rabbit anti- ATG4B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ATG4B at approximately 48-50KD. The expected band size for ATG4B is at 44KD.

• 

  Flow Cytometry analysis of RT4 cells using anti-ATG4B antibody (A02885-2).
  Overlay histogram showing RT4 cells stained with A02885-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4B Antibody (A02885-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.