Anti-EGFR Antibody (Clone#OTI2G8)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF

M00023-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-EGFR antibody (M00023-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-EGFR antigen affinity purified monoclonal antibody (M00023-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa.
all(2)
Anti-EGFR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EGFR .
all(2)

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产品名称

Anti-EGFR Antibody (Clone#OTI2G8)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

EGFR

克隆号

OTI2G8

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human EGFR(NP_958440) produced in HEK293T cell.

计算分子量

134 kDa

实际分子量

42.4 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Uniprot ID

P00533

文献引用格式

EGFR Antibody (Clone#OTI2G8) (Boster Biological Technology, Wuhan, China. Catalog#M00023-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

epidermal growth factor receptor

蛋白名全称

Epidermal growth factor receptor

实验方案>

实验步骤

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