Anti-EGFR (Phospho-Y1173) Antibody (Clone#EHG-5)

Rabbit Monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4453

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Phospho-EGFR (Y1197) expression in A431 cell lysate treated with EGF.
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IHC analysis of EGFR using anti-EGFR antibody (BM4453).
EGFR was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-EGFR Antibody (BM4453) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Immunofluorescent analysis using the Antibody.
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产品名称

Anti-EGFR (Phospho-Y1173) Antibody (Clone#EHG-5)

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Proto oncogene c ErbB 1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP

基因名称

EGFR

克隆号

EHG-5

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-EGFR (Y1173)

计算分子量

134 kDa

实际分子量

175 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Uniprot ID

P00533

Gene ID

1956

文献引用格式

EGFR (Phospho-Y1173) Antibody (Clone#EHG-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4453)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Epidermal growth factor receptor

实验方案>

实验步骤

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