产品名称
Anti-Alpha Internexin/INA Antibody (Clone#AECG-9)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
66 kDa neurofilament protein; Alpha internexin; Alpha Inx; INA; NEF5; Neurofilament 5; NF 66; NF-66; TXBP 1
应用范围
WB, IHC, ICC/IF, IP, FCM
免疫原
A synthesized peptide derived from human alpha Internexin Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt，-20℃ as supplied.
背景资料
Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.
文献引用格式
Alpha Internexin/INA Antibody (Clone#AECG-9) (Boster Biological Technology, Wuhan, China. Catalog#BM5524)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:50 |
| Flow Cytometry (FCM): | 1:20 |
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