| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |

Western blot analysis of anti-ATG16L1 antibody (BM5177). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: rat C6 tissue lysates,
Lane 6: mouse NIH/3T3 tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG16L1 antigen affinity purified monoclonal antibody (BM5177) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG16L1 at approximately 68 kDa. The expected band size for ATG16L1 is at 68 kDa.

Western blot analysis of anti-ATG16L1 antibody (BM5177). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: rat C6 tissue lysates,
Lane 6: mouse NIH/3T3 tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG16L1 antigen affinity purified monoclonal antibody (BM5177) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG16L1 at approximately 68 kDa. The expected band size for ATG16L1 is at 68 kDa.


