Anti-BAK/BAK1 Antibody (Clone#CHI-2)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4247

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-BAK/BAK1 antibody (BM4247). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAK/BAK1 antigen affinity purified monoclonal antibody (BM4247) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAK/BAK1 at approximately 23-25 kDa. The expected band size for BAK/BAK1 is at 23 kDa.
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产品简介>

产品名称

Anti-BAK/BAK1 Antibody (Clone#CHI-2)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Apoptosis regulator BAK; BAK; BAK LIKE; BAK1; CDN1

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

BAK1

克隆号

CHI-2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Bak

计算分子量

23 kDa

实际分子量

23-25 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Uniprot ID

Q16611

文献引用格式

BAK/BAK1 Antibody (Clone#CHI-2) (Boster Biological Technology, Wuhan, China. Catalog#BM4247)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Bcl-2 homologous antagonist/killer

实验方案>

实验步骤

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