Anti-PKC Gamma/PRKCG Antibody (Clone#MC5)

Mouse monoclonal antibody

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筛选器： All WB IHC

BM0401

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- PKCG antibody (BM0401). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: SH-SY5Y whole cell lysates,
Lane 4: U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 tissue lysates,
Lane 7: mouse brain tissue lysates.
Use mouse anti- PKCG 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PKCG at approximately 80KD. The expected band size for PKCG is at 78KD.
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产品名称

Anti-PKC Gamma/PRKCG Antibody (Clone#MC5)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

PKC gamma; PKCC; PKCG; PRKCG; Protein kinase C gamma type; protein kinase C; gamma; SCA14

产品类型

Monoclonal

检验物种

human, mouse, rat, rabbit

应用范围

WB, IHC

基因名称

PRKCG

克隆号

MC5

宿主

Mouse

抗体亚型

IgG2a

免疫原

Polypeptide

计算分子量

78 kDa

实际分子量

80 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The gamma isotype of protein kinase C (PKC gamma) is a member of the classical PKC (cPKC) subfamily which is activated by Ca(2+) and diacylglycerol in the presence of phosphatidylserine. Physiologically, PKC gamma is activated by a mechanism coupled with receptor-mediated breakdown of inositol phospholipid as other cPKC isotypes such as PKC alpha and PKC beta. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, while PKC alpha and PKC beta are expressed in many tissues in addition to the brain. Within the brain, PKC gamma is the most abundant in the cerebellum, hippocampus and cerebral cortex, where the existence of neuronal plasticity has been demonstrated. PKC gamma gene is mutated in spinocerebellar ataxia type 14 (SCA14). Verbeek et al. (2005) point out the specific alterations in mutant PKC gamma function that could lead to the selective neuronal degeneration of SCA14.

Uniprot ID

P63319

文献引用格式

PKC Gamma/PRKCG Antibody (Clone#MC5) (Boster Biological Technology, Wuhan, China. Catalog#BM0401)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Protein kinase C gamma type

实验方案>

实验步骤

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