Western blot analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: C6 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Gamma/PRKCG antigen affinity purified polyclonal antibody (A01890) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Gamma/PRKCG at approximately 80 kDa. The expected band size for PKC Gamma/PRKCG is at 78 kDa.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse cerebellum tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). PKC gamma was detected in an immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(10) 
IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). PKC gamma was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Fluoro488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(10) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: C6 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Gamma/PRKCG antigen affinity purified polyclonal antibody (A01890) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Gamma/PRKCG at approximately 80 kDa. The expected band size for PKC Gamma/PRKCG is at 78 kDa.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse cerebellum tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). PKC gamma was detected in an immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). PKC gamma was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Fluoro488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: C6 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Gamma/PRKCG antigen affinity purified polyclonal antibody (A01890) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Gamma/PRKCG at approximately 80 kDa. The expected band size for PKC Gamma/PRKCG is at 78 kDa.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of mouse cerebellum tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of PKC Gamma/PRKCG using anti-PKC Gamma/PRKCG antibody (A01890).
PKC Gamma/PRKCG was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PKC Gamma/PRKCG Antibody (A01890) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). PKC gamma was detected in an immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of PKC gamma using anti-PKC gamma antibody (A01890). PKC gamma was detected in a paraffin-embedded section of rat celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKC gamma Antibody (A01890) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Fluoro488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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