Anti-SOX2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3292

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SOX2 using anti-SOX2 antibody (BA3292). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U251 whole cell lysates,
Lane 2: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX2 antigen affinity purified polyclonal antibody (BA3292) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX2 at approximately 34 kDa. The expected band size for SOX2 is at 34 kDa.
all(4)
IHC analysis of SOX2 using anti-SOX2 antibody (BA3292).
SOX2 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOX2 Antibody (BA3292) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of SOX2 using anti-SOX2 antibody (BA3292).
SOX2 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOX2 Antibody (BA3292) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of SOX2 using anti-SOX2 antibody (BA3292).
SOX2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOX2 Antibody (BA3292) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-SOX2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ANOP3; MCOPS3; SOX2; Transcription factor SOX 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

SOX2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human SOX2, different from the related mouse and rat sequence by one amino acid.

计算分子量

34 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SRY(sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential for maintaining self-renewal, or pluripotency of undifferentiated embryonic stem cells. Sox2 is a member of the Sox family of transcription factors, which have been shown to play key roles in many stages of mammalian development. This gene is mapped to 3q26.33. It is found that SOX2 can regulate OCT3/4 expression and maintains ES pluripotency through upstream transcription factors. SOX2 is identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinoma. In addition to those, SOX2 has a critical role in maintenance of embryonic and neural stem cells and holds great promise in research involving induced pluripotency, an emerging and very promising field of regenerative medicine.

Uniprot ID

P48431

文献引用格式

SOX2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3292)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SRY(sex determining region Y)-box 2

蛋白名全称

Transcription factor SOX-2

实验方案>

实验步骤

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