| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of anti-SOX2 antibody (A00105-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX2 antigen affinity purified polyclonal antibody (A00105-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.

Western blot analysis of anti-SOX2 antibody (A00105-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOX2 antigen affinity purified polyclonal antibody (A00105-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.


