| Western blot (WB): | 1:500-2000 |

Western blot analysis of CDK7 using anti-CDK7 antibody (BA1521-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat testis tissue lysates,
Lane 2: Rat ovary tissue lysates,
Lane 3: HELA whole cell lysates,
Lane 4: MCF-7 whole cell lysates,
Lane 5: A549 whole cell lysates,
Lane 6: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK7 antigen affinity purified polyclonal antibody (BA1521-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK7 at approximately 39 kDa. The expected band size for CDK7 is at 39 kDa.

Western blot analysis of CDK7 using anti-CDK7 antibody (BA1521-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat testis tissue lysates,
Lane 2: Rat ovary tissue lysates,
Lane 3: HELA whole cell lysates,
Lane 4: MCF-7 whole cell lysates,
Lane 5: A549 whole cell lysates,
Lane 6: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDK7 antigen affinity purified polyclonal antibody (BA1521-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDK7 at approximately 39 kDa. The expected band size for CDK7 is at 39 kDa.
