Anti-CD95/FAS Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA0484

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD95/FAS using anti-CD95/FAS antibody (BA0484). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat cardiac muscle tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD95/FAS antigen affinity purified polyclonal antibody (BA0484) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD95/FAS at approximately 50 kDa. The expected band size for CD95/FAS is at 37 kDa.
all(3)
Western blot analysis of CD95/FAS using anti-CD95/FAS antibody (BA0484). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD95/FAS antigen affinity purified polyclonal antibody (BA0484) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD95/FAS at approximately 50 kDa. The expected band size for CD95/FAS is at 37 kDa.
all(3)
IHC analysis of CD95/FAS using anti-CD95/FAS antibody (BA0484).
CD95/FAS was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD95/FAS Antibody (BA0484) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CD95/FAS Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ALPS1A; APO 1; Apo 1 antigen; APT1; CD95; FAS; FAS/CD95; FAS1; FASLG receptor; FASTM; TNFRSF6

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC

基因名称

FAS

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas.

计算分子量

37 kDa

实际分子量

50 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Uniprot ID

Q63199

文献引用格式

CD95/FAS Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0484)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Fas(TNF receptor superfamily, member 6)

蛋白名全称

Tumor necrosis factor receptor superfamily member 6

实验方案>

实验步骤

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