Anti-CD95/FAS Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of CD95/FAS using anti-CD95/FAS antibody (A00055). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat thymus tissue lysates,
  Lane 2: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD95/FAS antigen affinity purified polyclonal antibody (A00055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD95/FAS at approximately 37,50 kDa. The expected band size for CD95/FAS is at 37 kDa.

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  Flow Cytometry analysis of mouse spleen tissue using anti-CD95/FAS antibody (A00055).
  Overlay histogram showing mouse spleen tissue stained with A00055 (Blue line). The tissue was blocked with 10% normal goat serum. And then incubated with rabbit anti-CD95/FAS Antibody (A00055) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Raw264.7 cells using anti-CD95/FAS antibody (A00055).
  Overlay histogram showing Raw264.7 cells stained with A00055 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD95/FAS Antibody (A00055) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of CD95/FAS using anti-CD95/FAS antibody (A00055). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat thymus tissue lysates,
  Lane 2: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD95/FAS antigen affinity purified polyclonal antibody (A00055) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD95/FAS at approximately 37,50 kDa. The expected band size for CD95/FAS is at 37 kDa.

• 

  Flow Cytometry analysis of mouse spleen tissue using anti-CD95/FAS antibody (A00055).
  Overlay histogram showing mouse spleen tissue stained with A00055 (Blue line). The tissue was blocked with 10% normal goat serum. And then incubated with rabbit anti-CD95/FAS Antibody (A00055) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of Raw264.7 cells using anti-CD95/FAS antibody (A00055).
  Overlay histogram showing Raw264.7 cells stained with A00055 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD95/FAS Antibody (A00055) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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