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Anti-MCT1/SLC16A1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A02240-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of MCT1/SLC16A1 using anti-MCT1/SLC16A1 antibody (A02240-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human U87 whole cell lysates,
    Lane 2: human HL-60 whole cell lysates,
    Lane 3: human K562 whole cell lysates,
    Lane 4: human 293T whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCT1/SLC16A1 antigen affinity purified polyclonal antibody (A02240-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MCT1/SLC16A1 at approximately 40 kDa. The expected band size for MCT1/SLC16A1 is at 54 kDa.

    all(4)
  • IHC analysis of MCT1/SLC16A1 using anti-MCT1/SLC16A1 antibody (A02240-1) .
    MCT1/SLC16A1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-MCT1/SLC16A1 Antibody (A02240-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(4)
  • IHC analysis of MCT1/SLC16A1 using anti-MCT1/SLC16A1 antibody (A02240-1) .
    MCT1/SLC16A1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-MCT1/SLC16A1 Antibody (A02240-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(4)

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产品名称
Anti-MCT1/SLC16A1 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
HHF7; MCT; MCT 1; MCT1; Monocarboxylate transporter 1; SLC16A1
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ELISA
基因名称
SLC16A1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human MCT1/SLC16A1 recombinant protein (Position: R116-V500).
计算分子量
54 kDa
实际分子量
40 kDa
成分
500 ug/ml antibody with PBS,0.02% NaN3, 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Uniprot ID
P53985  
文献引用格式
MCT1/SLC16A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02240-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
基因名全称
solute carrier family 16, member 1(monocarboxylic acid transporter 1)
蛋白名全称
Monocarboxylate transporter 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-MCT1/SLC16A1 Antibody

    筛选器: All WB IHC ELISA

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