Anti-Cyclin D3/CCND3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB9103

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cyclin D3/CCND3 using anti-Cyclin D3/CCND3 antibody (PB9103).
Lane 1: recombinant human Cyclin D3 protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin D3/CCND3 antigen affinity purified polyclonal antibody (PB9103) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin D3/CCND3 at approximately 25 kDa.
all(2)
Western blot analysis of Cyclin D3/CCND3 using anti-Cyclin D3/CCND3 antibody (PB9103). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat ovary tissue lysates,
Lane 5: JURKAT whole cell lysates,
Lane 6: A549 whole cell lysates,
Lane 7: MCF-7 whole cell lysates,
Lane 8: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin D3/CCND3 antigen affinity purified polyclonal antibody (PB9103) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin D3/CCND3 at approximately 33 kDa. The expected band size for Cyclin D3/CCND3 is at 33 kDa.
all(2)

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产品简介>

产品名称

Anti-Cyclin D3/CCND3 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CCND3; cyclin D; Cyclin D3; G1/S specific cyclin D3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

CCND3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Cyclin D3 recombinant protein (Position: Q136-L292). Human Cyclin D3 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat Cyclin D3, respectively.

计算分子量

33 kDa

实际分子量

33 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CCND3, also called Cyclin D3, is a protein that in humans is encoded by the CCND3 gene. It is mapped to 6p21.1. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. In addition, overexpression of CCND3 upregulated the translational activity in HeLa cells in a dose-dependent manner.

Uniprot ID

P30281

RRID

AB_3081970

文献引用格式

Cyclin D3/CCND3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9103)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin D3

蛋白名全称

G1/S-specific cyclin-D3

实验方案>

实验步骤

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