Western blot analysis of ABHD5 using anti-ABHD5 antibody (PB10021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were traABHD5erred to a membrane. Then the membrane was incubated with rabbit anti-ABHD5 antigen affinity purified polyclonal antibody (PB10021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The siABHD5 is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABHD5 at approximately 39 kDa. The expected band size for ABHD5 is at 39 kDa.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of ABHD5 using anti-ABHD5 antibody (PB10021) and anti-Alpha Tubulin antibody (M03989-3).
ABHD5 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of ABHD5 using anti-ABHD5 antibody (PB10021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were traABHD5erred to a membrane. Then the membrane was incubated with rabbit anti-ABHD5 antigen affinity purified polyclonal antibody (PB10021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The siABHD5 is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABHD5 at approximately 39 kDa. The expected band size for ABHD5 is at 39 kDa.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of ABHD5 using anti-ABHD5 antibody (PB10021) and anti-Alpha Tubulin antibody (M03989-3).
ABHD5 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
Western blot analysis of ABHD5 using anti-ABHD5 antibody (PB10021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were traABHD5erred to a membrane. Then the membrane was incubated with rabbit anti-ABHD5 antigen affinity purified polyclonal antibody (PB10021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The siABHD5 is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABHD5 at approximately 39 kDa. The expected band size for ABHD5 is at 39 kDa.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of ABHD5 using anti-ABHD5 antibody (PB10021).
ABHD5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
ICC/IF analysis of ABHD5 using anti-ABHD5 antibody (PB10021) and anti-Alpha Tubulin antibody (M03989-3).
ABHD5 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABHD5 Antibody (PB10021) at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) (Catalog # BA1031) were used as secondary antibody.
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