Anti-Cyclin A/CCNA1 Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of Cyclin A/CCNA1 using anti-Cyclin A/CCNA1 antibody (PB0515). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: rat kidney tissue lysates,
  Lane 5: rat testis tissue lysates,
  Lane 6: mouse kidney tissue lysates,
  Lane 7: mouse testis tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin A/CCNA1 antigA03957-Aen affinity purified polyclonal antibody (PB0515) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin A/CCNA1 at approximately 52 kDa. The expected band size for Cyclin A/CCNA1 is at 52 kDa.

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  ICC/IF analysis of Cyclin A/CCNA1 using anti-Cyclin A/CCNA1 antibody (PB0515) and anti-Alpha Tubulin antibody (M03989-3).
  Cyclin A/CCNA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Cyclin A/CCNA1 Antibody (PB0515) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green) (Catalog # BA1101) were used as secondary antibody.

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  Flow Cytometry analysis of PC-3 cells using anti-Cyclin A/CCNA1 antibody (PB0515).
  Overlay histogram showing PC-3 cells stained with PB0515 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A/CCNA1 Antibody (PB0515) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of Cyclin A/CCNA1 using anti-Cyclin A/CCNA1 antibody (PB0515). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: rat kidney tissue lysates,
  Lane 5: rat testis tissue lysates,
  Lane 6: mouse kidney tissue lysates,
  Lane 7: mouse testis tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin A/CCNA1 antigA03957-Aen affinity purified polyclonal antibody (PB0515) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin A/CCNA1 at approximately 52 kDa. The expected band size for Cyclin A/CCNA1 is at 52 kDa.

• 

  ICC/IF analysis of Cyclin A/CCNA1 using anti-Cyclin A/CCNA1 antibody (PB0515) and anti-Alpha Tubulin antibody (M03989-3).
  Cyclin A/CCNA1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Cyclin A/CCNA1 Antibody (PB0515) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green) (Catalog # BA1101) were used as secondary antibody.

• 

  Flow Cytometry analysis of PC-3 cells using anti-Cyclin A/CCNA1 antibody (PB0515).
  Overlay histogram showing PC-3 cells stained with PB0515 (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A/CCNA1 Antibody (PB0515) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.