Anti-ACAT2 Antibody (Clone#OTI3A11)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of anti-ACAT2 antibody (M03245-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human HT-29 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human Jurkat whole cell lysates,
  Lane 6: human MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACAT2 antigen affinity purified monoclonal antibody (M03245-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACAT2 at approximately 41 kDa. The expected band size for ACAT2 is at 41 kDa.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody.

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  Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 .

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  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.

• 

  Western blot analysis of anti-ACAT2 antibody (M03245-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human HT-29 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human Jurkat whole cell lysates,
  Lane 6: human MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACAT2 antigen affinity purified monoclonal antibody (M03245-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACAT2 at approximately 41 kDa. The expected band size for ACAT2 is at 41 kDa.

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

• 

  Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody.

• 

  Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 .

• 

  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.