指标别名
betacellulin; BTC; Probetacellulin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human BTC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0980)
背景资料
Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0980-CAP | 96-well plate precoated with anti- human Betacellulin antibody | 1 |
| EK0980-ST | Lyophilized recombinant human Betacellulin standard | 10ng/tubex2 |
| EK0980-DA | Biotinylated anti- human Betacellulin antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 220 | 402 | 34 | 202 | 396 |
| Standard deviation | 1.47 | 12.76 | 26.13 | 1.66 | 15.15 | 30.88 |
| CV(%) | 4.1 | 5.8 | 6.5 | 4.9 | 7.5 | 7.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.007 | 0.087 | 0.159 | 0.313 | 0.541 | 0.913 | 1.508 | 2.266 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)