Anti-ULK1 Antibody (Clone#ABHH-21)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM5170

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ULK1 antibody (BM5170). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ULK1 antigen affinity purified monoclonal antibody (BM5170) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ULK1 at approximately 130 kDa. The expected band size for ULK1 is at 113 kDa.
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IHC analysis of ULK1 using anti-ULK1 antibody (BM5170) .
ULK1 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-ULK1 Antibody (BM5170) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ULK1 using anti-ULK1 antibody (BM5170) .
ULK1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ULK1 Antibody (BM5170) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ULK1 using anti-ULK1 antibody (BM5170) .
ULK1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ULK1 Antibody (BM5170) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ULK1 using anti-ULK1 antibody (BM5170) .
ULK1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ULK1 Antibody (BM5170) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ULK1 Antibody (Clone#ABHH-21)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ATG1; KIAA0722; ULK1; Unc 51 like kinase 1; UNC51; Unc51.1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

ULK1

克隆号

ABHH-21

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ULK1

计算分子量

113 kDa

实际分子量

130-150 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ULK1 is an enzyme that in humans is encoded by the ULK1 gene. It is mapped to 12q24.33. Unc-51 like autophagy activating kinase (ULK1/2) are two similar isoforms of an enzyme that in humans are encoded by the ULK1/2 genes. It is specifically a kinase that is involved with autophagy, particularly in response to amino acid withdrawal. Not many studies have been done comparing the two isoforms, but some differences have been recorded.

Uniprot ID

O75385

文献引用格式

ULK1 Antibody (Clone#ABHH-21) (Boster Biological Technology, Wuhan, China. Catalog#BM5170)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Serine/threonine-protein kinase ULK1

实验方案>

实验步骤

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