| Western blot (WB): | 1:500-2000 |
| ImmunoPrecipitation (IP): | 1:20 |

Western blot analysis of anti-ATG10 antibody (BM5104). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG10 antigen affinity purified monoclonal antibody (BM5104) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG10 at approximately 28 kDa. The expected band size for ATG10 is at 25 kDa.

Immunoprecipitate (IP) analysis using the Antibody. (wb)

Western blot analysis of anti-ATG10 antibody (BM5104). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG10 antigen affinity purified monoclonal antibody (BM5104) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG10 at approximately 28 kDa. The expected band size for ATG10 is at 25 kDa.

Immunoprecipitate (IP) analysis using the Antibody. (wb)

