Anti-PAX6 Antibody (Clone#HGF-16)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4746

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-PAX6 antibody (BM4746). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Hela whole cell lysates Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAX6 antigen affinity purified monoclonal antibody (BM4746) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PAX6 at approximately 49 kDa. The expected band size for PAX6 is at 47 kDa.
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IHC analysis of PAX6 using anti-PAX6 antibody (BM4746) .
PAX6 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PAX6 Antibody (BM4746) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of PAX6 using anti-PAX6 antibody (BM4746) .
PAX6 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PAX6 Antibody (BM4746) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-PAX6 Antibody (Clone#HGF-16)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

AN; AN2; Aniridia type II protein; D11S812E; MGDA; Oculorhombin; paired box 6; Paired box protein Pax 6; PAX6; WAGR

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

PAX6

克隆号

HGF-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PAX6

计算分子量

47 kDa

实际分子量

47 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Paired box protein Pax-6, also known as aniridia type II protein (AN2) or oculorhombin, is a protein that in humans is encoded by the PAX6 gene. This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. In addition, this gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.

Uniprot ID

P26367

文献引用格式

PAX6 Antibody (Clone#HGF-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4746)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Paired box protein Pax-6

实验方案>

实验步骤

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