Anti-TrkB/NTRK2 (Phospho-Y817) Antibody (Clone#EGA-14)

Rabbit Monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4437

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-TrkB/NTRK2 antibody (BM4437). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TrkB/NTRK2 antigen affinity purified monoclonal antibody (BM4437) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TrkB/NTRK2 at approximately 130 kDa. The expected band size for TrkB/NTRK2 is at 92 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse brain cancer, using Phospho-TrkB (Y817) Antibody.
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Immunofluorescent analysis using the Antibody.
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产品简介>

产品名称

Anti-TrkB/NTRK2 (Phospho-Y817) Antibody (Clone#EGA-14)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

GP145 TrkB; NTRK2; TrkB; TrkB tyrosine kinase

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

NTRK2

克隆号

EGA-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-TrkB (Y817)

计算分子量

92 kDa

实际分子量

92,120-140 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TrkB is also known as NTRK2. This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. Tropomyosin receptor kinase B is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The neurotrophins that activate TrkB are: BDNF (Brain Derived Neurotrophic Factor), neurotrophin-4 (NT-4), and neurotrophin-3 (NT-3). As such, TrkB mediates the multiple effects of these neurotrophic factors, which includes neuronal differentiation and survival.

Uniprot ID

Q16620

文献引用格式

TrkB/NTRK2 (Phospho-Y817) Antibody (Clone#EGA-14) (Boster Biological Technology, Wuhan, China. Catalog#BM4437)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

BDNF/NT-3 growth factors receptor

实验方案>

实验步骤

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